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KMID : 1189420180310010012
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2018 Volume.31 No. 1 p.12 ~ p.21
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Effects of Naetakcheongeum-san on Anti-inflammatory Activities in RAW 264.7 cells
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Kim Tae-Jun
Kim Yong-Min
Kim Hee-Taek
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Abstract
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Objectives : Inflammation is one of the self-protective abilities against tissue injury, and it has clinical symptoms like redness, heat, swelling, pain, and loss of function. The purpose of this study is to examine inhibitory effects of Naetakchunkeum-san (NTCKS) on nitric oxide (NO), Prostaglandin E2 (PGE2), inducible NOS (iNOS), cyclooxygenase-2 (COX-2), and phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2), which play a major role in inflammatory response.
Methods : The experiment was performed using Raw 264.7 cells pretreated with NTCKS extracts. Cell viability was determined by MTT assay. To evaluate anti-inflammatory effects of NTCKS, we examined NO and PGE2 production in LPS-induced macrophages. We also investigated effects of NTCKS on iNOS, Cox-2, and ERK1/2 expression using western blot.
Results : In MTT assay, no cytotoxicity of NTCKS (50, 100, 150, 200㎍/㎖) on RAW 264.7 cell was found. LPS-induced NO production was decreased after treatment with NTCKS (150, 200㎍/㎖)(p<0.05). PGE2 was decreased after treatment with NTCKS (150, 200㎍/㎖)(p<0.05). NTCKS inhibited LPS-induced expressions of iNOS and COX-2 in a dose-dependent manner. Increased phosphorylation of ERK1/2 by LPS was decreased by NTCKS in a dose-dependent manner.
Conclusions : According to above experiments, NTCKS may be applied to inflammatory diseases such as atopic dermatitis, rheumatoid arthritis, and inflammatory bowel disease.
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KEYWORD
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Naetakchunkeum-san, Anti-inflammation, PGE2, iNOS, COX-2, ERK1/2
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